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3M's new molecular tests for STEC gives food safety labels more options

Arrival of two distinct assays for Shiga toxin-producing E. coli (STEC) enables food safety labs to test with or without eae gene results

3M STEC and coconut assays
3M STEC and coconut assays
3M STEC and coconut assays
3M STEC and coconut assays
August 29, 2019

3M Food Safety has announced the introduction of two assays that can detect the genes associated with Shiga toxin-producing E. coli (STEC), organisms that can pose severe health threats. The 3M Molecular Detection Assay 2 - STEC Gene Screen (stx and eae) is able to rapidly detect the genes for Shiga toxin types 1 and 2, as well as eae, the intimin gene that allows the bacteria to attach to intestinal cells. The second assay, the 3M Molecular Detection Assay 2 - STEC Gene Screen (stx) detects only Shiga toxin genes, allowing labs to serve varying screening needs. 

Both test kits are immediately available and are applicable to samples enriched from foods and from food processing environments. In addition, the assays have received Performance Tested Methods (PTM) certification from the AOAC Research Institute based on validation research conducted by independent laboratories.The 3M Molecular Detection Assay 2 - STEC Gene Screen (stx and eae) (Certificate #071902) demonstrated equivalent performance to the USDA FSIS method for raw beef trim and raw ground beef, and to the FDA BAM reference method for fresh spinach. The 3M Molecular Detection Assay 2 - STEC Gene Screen (stx) (Certificate #071903) demonstrated equivalent performance to the USDA FSIS method for raw ground beef, and to the FDA BAM reference method for fresh spinach. 

The new assays bring the number of next generation test kits compatible with the award-winning 3M Molecular Detection System to eight, three of which now relate to E. coli. The 3M Molecular Detection Assay 2 - E. coli O157 (including H7) was introduced in 2016 as a method for detecting the strain most commonly associated with E. coli O157 outbreaks. However, many manufacturers still wish to screen for non-O157 STECs, including eae-negative subsets like the O104:H4 strain that was responsible for a large outbreak in Europe earlier this decade.

“Pathogen testing isn’t a one-size-fits-all proposition so our team has worked diligently to give food processors a number of test kits to address the needs and regulations that may be uniquely confronting them,” said Caroline Michael, 3M Food Safety new product marketer. “We’re proud to offer a stx-only assay, as we know there are food manufacturers who want flexibility to test for a specific genetic profile. Flexibility is also enhanced with the (stx and eae) test kit, which clearly indicates the presence or absence of each of these genes in a test sample. For example, some companies want to rule out the presence of enteropathogenic E. coli – isolates that contain the eae gene but not stx– when interpreting results.”

The award-winning 3M Molecular Detection System platform and its various assays are used by food processors, universities, governments and contract testing laboratories around the world as a fast, accurate, easy-to-use and affordable test method. It overcomes limitations of conventional PCR (Polymerase Chain Reaction)  by using progressive loop-mediated isothermal amplification (LAMP) to amplify DNA sequences with high specificity and sensitivity, and then combines this process with bioluminescence as a means for detection. Presumptive positive results are revealed in real-time while negative results display once the test complete. In addition, the 3M Molecular Detection System is a primary method of the USDA FSIS for the detection of Salmonella and Listeria.

3M Food Safety helps the food and beverage industries optimize the quality and safety of their products to enable consumer protection. It provides solutions that mitigate risk, improve operational efficiencies and impact bottom lines. For more information on either the 3M Molecular Detection Assay 2 - STEC Gene Screen (stx and eae), 3M Molecular Detection Assay 2 - STEC Gene Screen (stx) and their AOAC-PTM certifications, visit www.3M.com/STEC.

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KEYWORDS: 3M Food Safety E. coli

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