Streamlined Microbiological Workflows for the Detection of Listeria monocytogenes in Food
Listeriosis, which is caused by eating food contaminated with the bacterium Listeria monocytogenes, is an important public health concern in the U.S. The U.S. Centers for Disease Control and Prevention (CDC) estimates that approximately 1,600 illnesses and 260 deaths occur annually due to listeriosis in the U.S.[1]
To ensure consumer safety, rapid detection methods of L. monocytogenes in food are critical. However, traditional methods for detection require an enrichment step that is time consuming and labor intensive.
This article describes a solution designed to simplify and shorten the workflow of pathogen enrichment. Readybag® Half Fraser Broth comprises aluminum pouches filled with preweighed and gamma-irradiated granulated culture media, eliminating the need for upfront media preparation. Readybag Half Fraser Broth is compliant with ISO standard formulation.
The article will evaluate the application of the enrichment media in Readybag pouches for the detection of pathogens in wiener sausages, cream cheese, cantaloupe melon and cooked prawns. The article will provide the workflow and data for L. monocytogenes detection according to ISO 11290-1 in detail. Traditional preparation of Half Fraser Broth was used as the reference method.
Materials and Equipment
Culture media:
• Readybag Half Fraser Broth 12.5 g (1.02449.0060)